ONOUE K, YAGI Y, STELOS P, PRESSMAN D
Science. 1964 Oct 16;146(3642):404-5. doi: 10.1126/science.146.3642.404.
Direct evidence is provided for the antigen binding activity of the 6S subunits formed by reduction and alkylation of rabbit beta2-macroglobulin antibody. Binding activity of the subunits was clearly demonstrated by radioimmunoelectrophoresis with a preparation of purified rabbit antibody against the p-azobenzenearsonate group, which contained 40 percent of beta2M-antibody. The precipitate arc formed between the subunits and sheep antiserum against rabbit macroglobulin was shown to bind radioactive antigen specifically.
通过对兔β2-微球蛋白抗体进行还原和烷基化形成的6S亚基的抗原结合活性提供了直接证据。用针对对氨基苯砷酸基团的纯化兔抗体制剂进行放射免疫电泳，清楚地证明了这些亚基的结合活性，该制剂含有40%的β2M-抗体。亚基与抗兔巨球蛋白的绵羊抗血清之间形成的沉淀弧显示出能特异性结合放射性抗原。
Zotero 插件