Delhanty J J, Solomon J B
Immunology. 1966 Aug;11(2):103-13.
The maturation of the immune response to goat erythrocytes in chicken from hatching onwards is described. Agglutinins in immune sera were examined for their sedimentation properties, heat stability at 65°, resistance to 2-mercaptoethanol (ME) and ability to agglutinate at 4° or 37°. Large doses of goat erythrocytes were given to favour the induction of the IgG response. Agglutinins were tentatively classified as class I, which were cold and warm agglutinins, IgM, ME-susceptible and heat-labile; class II, a warm agglutinin, IgG, ME-resistant and only slightly heat-labile; and class III, a cold agglutinin, possibly IgA, partially resistant to ME but completely heat stable. Immune agglutinin was first detected in sera from 11-day-old chicks and all three types were detectable at 17 days of age. Multiple injections of goat erythrocytes induced an earlier response than single injections. Agglutination titres, in response to weight-adjusted doses of antigen, increased from 5 to 24 weeks of age. Chicks could be made hyperimmune by 6 weeks of age when the proportion of class II antibody was much increased compared to that in primary sera.
描述了雏鸡自孵化起对山羊红细胞免疫反应的成熟过程。检测了免疫血清中的凝集素的沉降特性、65°时的热稳定性、对2-巯基乙醇（ME）的抗性以及在4°或37°时的凝集能力。给予大剂量的山羊红细胞以促进IgG反应的诱导。凝集素被初步分类为：I类，即冷凝集素和温凝集素，IgM，对ME敏感且热不稳定；II类，一种温凝集素，IgG，对ME有抗性且仅略微热不稳定；III类，一种冷凝集素，可能是IgA，对ME部分抗性但完全热稳定。在11日龄雏鸡的血清中首次检测到免疫凝集素，在17日龄时可检测到所有三种类型。多次注射山羊红细胞比单次注射诱导的反应更早。响应重量调整后的抗原剂量，凝集滴度在5至24周龄时增加。雏鸡在6周龄时可产生超免疫，此时II类抗体的比例与初次血清相比大幅增加。
