一种使用聚合酶链反应（PCR）筛选转化酵母菌落的简单快速方法。
A simple and rapid method for screening transformant yeast colonies using PCR.
作者信息
Liang Q, Richardson T
机构信息
Department of Food Science and Technology, University of California, Davis 95616.
出版信息
Biotechniques. 1992 Nov;13(5):730-2, 735.
PMID:1418974
Abstract
A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification.
摘要
本文描述了一种筛选转化酵母菌落的简单快速方法。在此方法中，从少量酵母细胞团中分离出微量质粒DNA；然后，通过PCR扩增检测每个酵母菌落中外源DNA的存在。
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