Jakschik B A, Harrington L F, Malaviya R
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Eicosanoids. 1992;5(1):39-43.
Stimulation of peritoneal cells from BALB/c, CBA/J or WBB6F1(-)+/+ mice with IgE/antigen caused the release of mast cell granules and leukotriene C4. No leukotriene formation was observed with peritoneal cells from mast cell-deficient WBB6F1-W/Wv mice. Mast cells (greater than 98% purity), separated on metrizamide gradients, did not synthesize detectable amounts of leukotriene C4 when challenged immediately after purification. Co-culture of the mast cells with 3T3 fibroblasts restored the capability of the mast cells to produce leukotrienes. Addition of IL-3 during culture enhanced the synthesis of this eicosanoid. The 5-lipoxygenase inhibitor A-63162 blocked the leukotriene formation. Western blot analysis confirmed the presence of 5-lipoxygenase in connective tissue mast cells. These experiments demonstrate that mouse peritoneal (connective tissue) mast cells can produce significant amounts of leukotrienes.
用IgE/抗原刺激BALB/c、CBA/J或WBB6F1( - ) + / +小鼠的腹膜细胞，可导致肥大细胞颗粒和白三烯C4的释放。在肥大细胞缺陷的WBB6F1 - W/Wv小鼠的腹膜细胞中未观察到白三烯的形成。通过密度梯度离心分离得到的肥大细胞（纯度大于98%），在纯化后立即受到刺激时，不会合成可检测量的白三烯C4。将肥大细胞与3T3成纤维细胞共培养可恢复肥大细胞产生白三烯的能力。培养过程中添加IL - 3可增强这种类花生酸的合成。5 - 脂氧合酶抑制剂A - 63162可阻断白三烯的形成。蛋白质免疫印迹分析证实结缔组织肥大细胞中存在5 - 脂氧合酶。这些实验表明，小鼠腹膜（结缔组织）肥大细胞可产生大量白三烯。
