WHITBECK E G, ROSENBERG L T
Immunology. 1964 Jul;7(4):363-74.
The antigenic properties of guinea-pig spleen, liver and lymph node microsomes have been studied. These subcellular particles were isolated by differential centrifugation. Ribosomes were prepared by treating the microsomes with sodium desoxycholate. Double-diffusion in agar and immunoelectrophoresis were the principal serological techniques employed. The use of appropriately absorbed as well as unabsorbed antisera has permitted the recognition of spleen-specific antigens associated with membranous elements of the cell. A liver-specific antigen has also been found. Serum protein antigens associated with the micro-somes and a particle-specific antigen associated with ribosomes have been recognized. The microsomes from the three tissues contain the Forssman antigen. No evidence was obtained to indicate that the RNA present functioned as an antigenic determinant.
对豚鼠脾脏、肝脏和淋巴结微粒体的抗原特性进行了研究。这些亚细胞颗粒通过差速离心法分离。通过用脱氧胆酸钠处理微粒体制备核糖体。琼脂双向扩散和免疫电泳是主要采用的血清学技术。使用适当吸收和未吸收的抗血清能够识别与细胞膜成分相关的脾脏特异性抗原。还发现了一种肝脏特异性抗原。已经识别出与微粒体相关的血清蛋白抗原和与核糖体相关的颗粒特异性抗原。来自这三种组织的微粒体含有福斯曼抗原。没有获得证据表明存在的RNA起到抗原决定簇的作用。
