DAVIS J B
Appl Microbiol. 1964 Jul;12(4):301-4. doi: 10.1128/am.12.4.301-304.1964.
Lipid fractions of propane- and n-butane-grown nocardial cells each contain a chloroform-soluble, ether-insoluble polymer not observed previously in liquid n-alkane-grown cells. The polymer in propane-grown cells is poly-beta-hydroxybutyrate. The polymer in n-butane-grown cells apparently contains unsaturation in the molecule, and is identified tentatively as a co-polymer of beta-hydroxybutyric and beta-hydroxybutenoic (specifically 3-hydroxy 2-butenoic) acids. The other major component of the lipid fraction consists of triglycerides containing principally palmitic and stearic acids. There seems to be little qualitative distinction in the glycerides of propane- or n-butane-grown cells. Oxidative assimilation of n-butane is described.
以丙烷和正丁烷培养的诺卡氏菌细胞的脂质组分各自含有一种氯仿可溶、乙醚不溶的聚合物,这是之前在液态正构烷烃培养的细胞中未观察到的。丙烷培养细胞中的聚合物是聚-β-羟基丁酸酯。正丁烷培养细胞中的聚合物分子中显然含有不饱和键,初步鉴定为β-羟基丁酸和β-羟基丁烯酸(具体为3-羟基-2-丁烯酸)的共聚物。脂质组分的其他主要成分是主要含有棕榈酸和硬脂酸的甘油三酯。丙烷或正丁烷培养细胞的甘油酯在性质上似乎没有太大区别。描述了正丁烷的氧化同化作用。