用于维持连续培养中瘤胃微生物种群的装置的改进
MODIFICATIONS OF A DEVICE FOR MAINTENANCE OF THE RUMEN MICROBIAL POPULATION IN CONTINUOUS CULTURE.
作者信息
SLYTER L L, NELSON W O, WOLIN M J
出版信息
Appl Microbiol. 1964 Jul;12(4):374-7. doi: 10.1128/am.12.4.374-377.1964.
Abstract
An improved and simplified apparatus for maintaining the rumen microbial population in continuous culture was constructed. All components were easily obtained from commercial sources or were simple to construct. Mechanical difficulties were minimal, and little attention was needed on the part of the operator. The deoxyribonucleic acid (DNA) content of the cultures (100 to 150 mug/ml) varied little during 7 days of continuous culture, and protozoal concentration decreased from 10(5) per ml to a steady-state level of 2 x 10(3) per ml in 4 days. Volatile fatty acid and methane production followed the normal in vivo pattern for 7 days of continuous culture. DNA, protozoal concentrations, and fermentation patterns did not significantly change between 4 and 21 days of continuous culture.
摘要
构建了一种用于在连续培养中维持瘤胃微生物群落的改进且简化的装置。所有组件都易于从商业渠道获得或易于构建。机械方面的困难极小，操作人员几乎无需关注。在连续培养的7天中，培养物的脱氧核糖核酸（DNA）含量（100至150微克/毫升）变化很小，原生动物浓度在4天内从每毫升10⁵降至每毫升2×10³的稳态水平。在连续培养的7天里，挥发性脂肪酸和甲烷的产生遵循正常的体内模式。在连续培养的4至21天之间，DNA、原生动物浓度和发酵模式没有显著变化。
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