RYTER A, LANDMAN O E
J Bacteriol. 1964 Aug;88(2):457-67. doi: 10.1128/jb.88.2.457-467.1964.
Ryter, Antoinette (Institut Pasteur, Paris, France), and Otto E. Landman. An electron microscope study of the relationship between mesosome loss and the stable L-state (or protoplast state) in Bacillus subtilis. J. Bacteriol. 88:457-467. 1964.-In a prior publication, it was postulated that inability of protoplasts to restart cell-wall synthesis and cell division and the inability of stable mass-conversion L forms to return to the bacillary state were both equivalent and both due to the interruption of a membrane-associated reaction sequence. It was further postulated that this reaction sequence might reside in the mesosome. In the present publication, it is shown by means of electron microscopy of thin sections that protoplasts and L forms do not contain mesosomes. The sequence of events leading to loss of the mesosomes during protoplasting is as follows. Soon after lysozyme addition, the mesosomes are extruded from the cell interior into the space between cell wall and cytoplasmic membrane. Mesosome fragments in the form of small vesicles gather at the poles of the cells and are released, along with intact protoplasts, when the wall fragments. (Sudden shift of bacilli to hypertonic environment also causes extrusion and fragmentation of mesosomes, but this damage is later repaired.) In intact bacilli, mesosomes are in contact with both the peripheral membrane and nuclear material. Upon extrusion of the mesosomes, a direct attachment between nuclear material and cytoplasmic membrane is observed. Deoxyribonucleic acid (DNA)-membrane attachment may play a role in the control of DNA replication. Bacillus subtilis L-colonies consist of irregularly shaped bodies of varying sizes, bounded only by a membrane. Many of the smaller bodies do not contain nuclear material, and many of the large ones appear inviable. Division is accomplished by a disorganized-appearing constriction process. There are no septa.
赖特，安托瓦内特（法国巴黎巴斯德研究所）和奥托·E·兰德曼。枯草芽孢杆菌中中间体丢失与稳定L态（或原生质体态）关系的电子显微镜研究。《细菌学杂志》88:457 - 467。1964年。——在之前的一篇出版物中曾推测，原生质体无法重新启动细胞壁合成和细胞分裂，以及稳定的大量转化L型无法恢复到杆菌状态，这两者是等效的，并且都是由于与膜相关的反应序列中断所致。进一步推测该反应序列可能存在于中间体中。在本出版物中，通过薄切片的电子显微镜观察表明，原生质体和L型不含有中间体。原生质体形成过程中导致中间体丢失的事件顺序如下。添加溶菌酶后不久，中间体从细胞内部被挤出到细胞壁和细胞质膜之间的空间。小泡形式的中间体片段聚集在细胞两极，并在细胞壁破裂时与完整的原生质体一起释放。（杆菌突然转移到高渗环境也会导致中间体挤出和碎片化，但这种损伤随后会得到修复。）在完整的杆菌中，中间体与外周膜和核物质都接触。中间体挤出后，观察到核物质与细胞质膜之间有直接附着。脱氧核糖核酸（DNA）-膜附着可能在DNA复制的控制中起作用。枯草芽孢杆菌L菌落由大小不一的不规则形状的物体组成，仅由一层膜界定。许多较小的物体不含核物质，许多较大的物体似乎无法存活。分裂是通过一种看起来杂乱无章的缢缩过程完成的。没有隔膜。
