从L和H多肽链重建抗噬菌体抗体及种间分子杂交体的形成

RECONSTITUTION OF ANTIPHAGE ANTIBODIES FROM L AND H POLYPEPTIDE CHAINS AND THE FORMATION OF INTERSPECIES MOLECULAR HYBRIDS.

作者信息

FOUGEREAU M, OLINS D E, EDELMAN G M

出版信息

J Exp Med. 1964 Sep 1;120(3):349-58. doi: 10.1084/jem.120.3.349.

DOI:10.1084/jem.120.3.349

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2137761/

Abstract

Reconstitution of phage-neutralizing activity was observed after admixture of separated L and H polypeptide chains of purified antibodies obtained from single sheep or guinea pigs and directed against f1 and f2 phages. Hybrid mixtures of complementary chains of antiphage antibodies and gamma-globulins of the same animal showed less activity than the homologous mixtures of chains from antibodies of the same specificity. 7S interspecies molecular hybrids could be formed between L or H chains of sheep gamma-globulins and the complementary chains of guinea pig gamma-globulins. In contrast to the results obtained within a single animal species, mixtures of L or H chains of antiphage antibodies from one species with the complementary chains of antibodies to the same phage from the other species showed slight or negligible potentiation of neutralizing activity.

摘要

从单只绵羊或豚鼠体内获得的、针对f1和f2噬菌体的纯化抗体，其分离的L和H多肽链混合后，可观察到噬菌体中和活性的重建。抗噬菌体抗体互补链与同一动物的γ球蛋白的杂交混合物，其活性低于相同特异性抗体链的同源混合物。绵羊γ球蛋白的L链或H链与豚鼠γ球蛋白的互补链之间可形成7S种间分子杂种。与在单一动物物种内获得的结果相反，来自一个物种的抗噬菌体抗体的L链或H链与来自另一物种针对同一噬菌体的抗体的互补链混合后，中和活性的增强轻微或可忽略不计。

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