Tao N J, Lindsay S M, Lees S
Department of Physics, Arizona State University, Tempe 85287.
Biophys J. 1992 Oct;63(4):1165-9. doi: 10.1016/S0006-3495(92)81692-2.
We have used the atomic force microscope (AFM) to measure the local rigidity modulus at points on the surface of a section of hydrated cow tibia. These data are obtained either from contrast changes that occur as the contact force is altered, or from force versus distance curves obtained at fixed points. These two methods yield the same values for rigidity modulus (at a given point). At low resolution, the elastic morphology and topography mirror the features seen in optical and electron micrographs. At high resolution we see dramatic variations in elastic properties across distances as small as 50 nm.
我们使用原子力显微镜（AFM）来测量一段水合牛胫骨表面各点的局部刚性模量。这些数据要么是通过改变接触力时发生的对比度变化获得的，要么是从在固定点获得的力与距离曲线中获得的。这两种方法（在给定点）得出的刚性模量值相同。在低分辨率下，弹性形态和地形反映了光学和电子显微镜图像中看到的特征。在高分辨率下，我们看到在小至50纳米的距离上弹性特性有显著变化。
