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关于转录起始中启动子的碱基序列。

On the base sequences of the promoters in transcription initiation.

作者信息

Shinoda T

机构信息

National Chemical Laboratory for Industry, Tsukuba Research Center, Ibaraki, Japan.

出版信息

Biophys Chem. 1992 Aug;44(1):61-70. doi: 10.1016/0301-4622(92)85035-3.

Abstract

The base sequence of a specific DNA region identified as the promoter is investigated by means of the quantity S(r) corresponding to "superdelocalizability" of oxygen ion of each phosphate for the ten DNA dimer units ([XY/Y'X']2-) and ([XY/Y'X']2- + H+)-complexes. A mechanism is proposed of how RNA polymerase can recognize its transcription site (phosphate), and is applied to the Escherichia coli promoters, lacUV5, recAp, rrnEpl, and rrnEp2. The result explains fairly well the character of the promoters experimentally found.

摘要

通过对应于十个DNA二聚体单元([XY/Y'X']2-)和([XY/Y'X']2- + H+)-复合物中每个磷酸根氧离子“超离域性”的量S(r),研究被鉴定为启动子的特定DNA区域的碱基序列。提出了一种RNA聚合酶如何识别其转录位点(磷酸根)的机制,并将其应用于大肠杆菌启动子lacUV5、recAp、rrnEpl和rrnEp2。该结果相当好地解释了实验发现的启动子的特性。

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