Berezhnoy Andrey Y, Shckorbatov Yuriy G
National Scientific Center, Kharkov Physical-Technical Institute, Kharkov 61108, Ukraine.
J Zhejiang Univ Sci B. 2005 Nov;6(11):1063-8. doi: 10.1631/jzus.2005.B1063.
The energy of interaction between complementary nucleotides in promoter sequences of E. coli was calculated and visualized. The graphic method for presentation of energy properties of promoter sequences was elaborated on. Data obtained indicated that energy distribution through the length of promoter sequence results in picture with minima at -35, -8 and +7 regions corresponding to areas with elevated AT (adenine-thymine) content. The most important difference from the random sequences area is related to -8. Four promoter groups and their energy properties were revealed. The promoters with minimal and maximal energy of interaction between complementary nucleotides have low strengths, the strongest promoters correspond to promoter clusters characterized by intermediate energy values.
计算并可视化了大肠杆菌启动子序列中互补核苷酸之间的相互作用能量。阐述了用于呈现启动子序列能量特性的图形方法。获得的数据表明,启动子序列长度上的能量分布导致在对应于富含AT(腺嘌呤 - 胸腺嘧啶)区域的 -35、-8和 +7区域出现能量最小值的图谱。与随机序列区域最重要的差异与 -8 相关。揭示了四个启动子组及其能量特性。互补核苷酸之间相互作用能量最小和最大的启动子强度较低,最强的启动子对应于具有中间能量值的启动子簇。