用于表皮葡萄球菌的噬菌体分型试剂盒的开发。
Development of a bacteriophage-typing set for Staphylococcus epidermidis.
作者信息
Skahan J M, Parisi J T
出版信息
J Clin Microbiol. 1977 Jul;6(1):16-8. doi: 10.1128/jcm.6.1.16-18.1977.
Six phages isolated from lysogenic cultures of Staphylococcus epidermidis were used to type cultures in our collection classified previously as "untypable" with our original typing set of 13 phages, new clinical isolates from Missouri, and clinical isolates from Connecticut, Georgia, Pennsylvania, and Virginia. Typability with the new set of 6 phages and with our 13 original phages was compared. For 203 cultures, 81 (39.9%) cultures were typable with the set of 6 phages and 73 (36.0%) cultures were typable with the set of 13 phages. When the two typing sets were combined, the percentage of typability for 425 cultures increased to 49.9%. compared with 35.7% with the original set of 13 phages. For 109 cultures from outstate, the percentage of typability was 56.9%, whereas that for 316 cultures from Missouri was 47.5%. When only cultures in biotype 1 were considered, the percentage of typability for 268 cultures with the combined set increased to 58.2%, compared with 39.5% with the original set of 13 phages. Analysis of individual reactions for all 425 cultures typed in our laboratory indicated that 11 of the 19 phages in the combined set accounted for the majority of the reactions, and a provisional set containing these 11 phages is proposed.
从表皮葡萄球菌溶原培养物中分离出的6种噬菌体，用于对我们收集的先前用我们最初的13种噬菌体分型集分类为“无法分型”的培养物、来自密苏里州的新临床分离株以及来自康涅狄格州、佐治亚州、宾夕法尼亚州和弗吉尼亚州的临床分离株进行分型。比较了新的6种噬菌体分型集和我们最初的13种噬菌体的分型能力。对于203份培养物，81份（39.9%）培养物可用6种噬菌体分型集分型，73份（36.0%）培养物可用13种噬菌体分型集分型。当将这两种分型集合并时，425份培养物的分型率提高到49.9%，而最初的13种噬菌体分型集的分型率为35.7%。对于来自州外的109份培养物，分型率为56.9%，而来自密苏里州的316份培养物的分型率为47.5%。当仅考虑生物型1中的培养物时，268份培养物用合并分型集的分型率提高到58.2%，而最初的13种噬菌体分型集的分型率为39.5%。对我们实验室分型的所有425份培养物的个体反应分析表明，合并分型集中的19种噬菌体中有11种占反应的大部分，并提出了一个包含这11种噬菌体的临时分型集。
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