MEYLE J S, KEMPF J E
Appl Microbiol. 1964 Sep;12(5):400-2. doi: 10.1128/am.12.5.400-402.1964.
There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially purified and suspended in buffered saline solution, it rapidly lost titer. Of seven protective agents recommended in the literature, only gum acacia seemed to demonstrate significant protection of purified virus against loss of viability. The temperature of thawing of phage suspended in broth did not affect its titer after freezing and storing in liquid nitrogen.
关于在液氮温度（-196℃）下保存病毒的报道很少。在本研究中，观察了影响噬菌体存活的因素。肉汤中的噬菌体裂解物在液氮中储存4周后效价没有损失。冷冻速率并不关键，但在氮蒸气中冷冻效果不佳。当病毒部分纯化并悬浮在缓冲盐溶液中时，其效价迅速降低。在文献中推荐的七种保护剂中，只有阿拉伯胶似乎对纯化病毒的活力丧失有显著的保护作用。悬浮在肉汤中的噬菌体在液氮中冷冻和储存后，解冻温度不影响其效价。
