FIELDS M L
Appl Microbiol. 1964 Sep;12(5):407-11. doi: 10.1128/am.12.5.407-411.1964.
Heat-shocking spores at 110 C in 20% sucrose solutions decreased the percentage of the rough variant in a mixed population (rough and smooth variants) of strain M. Heat-shocking spores of the rough variant of strain NCA 1518 in 20% sucrose produced a decline in the number which germinated, whereas the smooth variant of strain NCA 1518 increased in the number which germinated. By the use of phase microscopy and plate counts, from the same incubated spore suspension in distilled water, heat-induced dormancy was demonstrated at 52 C. Dormancy also occurred in 20% sucrose solutions when held at room temperatures. Heat-shocking spores of strain M in 20% sucrose solutions and plating immediately after 24- and 48-hr holding periods at 25 C produced a decline in the total population with the percentage of rough variant increasing with time. A second heat shock produced only an increase in the rough variant.
在20%蔗糖溶液中于110℃对菌株M的孢子进行热激处理，可降低混合群体（粗糙型和光滑型变体）中粗糙型变体的比例。在20%蔗糖中对菌株NCA 1518的粗糙型变体孢子进行热激处理，使其发芽数量减少，而菌株NCA 1518的光滑型变体发芽数量增加。通过相差显微镜和平板计数法，在蒸馏水中相同的培养孢子悬液中，证明了在52℃时热诱导休眠现象的存在。当置于室温下时，在20%蔗糖溶液中也会出现休眠现象。在20%蔗糖溶液中对菌株M的孢子进行热激处理，并在25℃下保持24小时和48小时后立即进行平板接种，结果显示总群体数量下降，粗糙型变体的比例随时间增加。第二次热激处理仅使粗糙型变体数量增加。
