MERGENHAGEN S E, MARTIN G R
J Bacteriol. 1964 Oct;88(4):1169-74. doi: 10.1128/jb.88.4.1169-1174.1964.
Mergenhagen, Stephan E. (National Institute of Dental Research, Bethesda, Md.), and George R. Martin. Properties of a lysozyme-dissociated endotoxic fraction from Escherichia coli. J. Bacteriol. 88:1169-1174. 1964.-Treatment of a phenol-water preparation of C(14)-labeled Escherichia coli O91-H21 endotoxin of low solubility with lysozyme at pH 5.0 or 8.0 effected a dissociation of the preparation. Such products of dissociation were equally distributed in the chloroform and water phases after extraction. beta-Glucosidase, but not beta-galactosidase, significantly dissociated this endotoxin also. Concomitant with dissociation, recoverable endotoxin after lysozyme treatment had a reduced content of bound lipid, and dissolved easily in aqueous media to yield a clear solution. Examination of lysozyme-treated endotoxin in an analytical ultracentrifuge revealed that it sedimented as a single major boundary with a sedimentation coefficient of 13.3. Lysozyme-treated endotoxin was more potent than was the conventional endotoxin as evidenced by lethal activity in rabbits and pertussis-sensitized mice. Agar-gel diffusion analysis indicated that the higher molecular weight component associated with conventional endotoxin was dissociated by lysozyme treatment. In immunoelectrophoresis, lysozyme-treated endotoxin was observed as a single sharp band of precipitation which migrated toward the cathode.
默根哈根，斯蒂芬·E.（国立牙科研究所，马里兰州贝塞斯达），以及乔治·R.马丁。大肠杆菌溶菌酶解离内毒素组分的性质。《细菌学杂志》88:1169 - 1174。1964年。——用溶菌酶在pH 5.0或8.0条件下处理低溶解度的C(14)标记的大肠杆菌O91 - H21内毒素的酚 - 水制剂，会使该制剂发生解离。提取后，这种解离产物在氯仿相和水相中均匀分布。β - 葡萄糖苷酶而非β - 半乳糖苷酶也能显著解离这种内毒素。伴随解离，溶菌酶处理后可回收的内毒素结合脂质含量降低，且易溶于水介质中形成澄清溶液。在分析超速离心机中检测溶菌酶处理后的内毒素，发现其以单一主要边界沉降，沉降系数为13.3。如在兔和百日咳致敏小鼠中的致死活性所示，溶菌酶处理后的内毒素比传统内毒素更具效力。琼脂凝胶扩散分析表明，与传统内毒素相关的高分子量组分经溶菌酶处理后发生解离。在免疫电泳中，溶菌酶处理后的内毒素表现为一条单一的尖锐沉淀带，向阴极迁移。
