一种重新聚焦和优化的HNCA：提高了对大型大分子的灵敏度和分辨率。
A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.
作者信息
Farmer B T, Venters R A, Spicer L D, Wittekind M G, Müller L
机构信息
Department of Research and Development, Varian Associates, Palo Alto, CA 94304.
出版信息
J Biomol NMR. 1992 Mar;2(2):195-202. doi: 10.1007/BF01875530.
PMID:1422152
Abstract
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
摘要
描述了一种三维优化、重新聚焦的HNCA实验。当应用于[13C, 15N]标记的人碳酸酐酶II（一种29 kDa的蛋白质）时，该实验的灵敏度显著提高。讨论了灵敏度提高的原因，并指出了三个有待进一步发展的不同领域。
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