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一种重新聚焦和优化的HNCA：提高了对大型大分子的灵敏度和分辨率。

A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.

作者信息

Farmer B T, Venters R A, Spicer L D, Wittekind M G, Müller L

机构信息

Department of Research and Development, Varian Associates, Palo Alto, CA 94304.

出版信息

J Biomol NMR. 1992 Mar;2(2):195-202. doi: 10.1007/BF01875530.

DOI:10.1007/BF01875530
PMID:1422152
Abstract

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

摘要

描述了一种三维优化、重新聚焦的HNCA实验。当应用于[13C, 15N]标记的人碳酸酐酶II（一种29 kDa的蛋白质）时，该实验的灵敏度显著提高。讨论了灵敏度提高的原因，并指出了三个有待进一步发展的不同领域。

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