Reidy J, Wright I, Boom S J, Barclay R, Di Padova F, Ramsay G
University Department of Surgery, Western Infirmary, Glasgow, UK.
Br J Surg. 1992 Oct;79(10):1087-90. doi: 10.1002/bjs.1800791033.
Passive immunization with antibody to the core region of endotoxin (core lipopolysaccharide (LPS)) has been reported to reduce mortality in severe sepsis. A rat model of endotoxaemia that reproduces the hyperdynamic cardiovascular state seen in early sepsis was developed to test monoclonal antibodies specific for core LPS. A thermodilution technique of measuring cardiac output was adapted for use in rats. Twenty-five animals were anaesthetized and mechanically ventilated with monitoring of central venous pressure and mean arterial pressure. Fluid replacement was adjusted to maintain the central venous pressure. Controls (n = 10) and antibody-treated animals (n = 5) showed no significant change in cardiac output. Animals given 0.1 mg kg-1 R2 endotoxin over 1 h (n = 5) showed a significant rise in cardiac output of 65 per cent (P < 0.01). This was abolished in rats given both antibody and endotoxin (n = 5). This study provides evidence that a monoclonal antibody against core LPS abolishes the hyperdynamic state induced by endotoxin infusion.
据报道，用针对内毒素核心区域（核心脂多糖（LPS））的抗体进行被动免疫可降低严重脓毒症的死亡率。为了测试针对核心LPS的单克隆抗体，建立了一种可重现早期脓毒症所见高动力心血管状态的大鼠内毒素血症模型。将一种测量心输出量的热稀释技术应用于大鼠。25只动物接受麻醉并进行机械通气，同时监测中心静脉压和平均动脉压。调整补液量以维持中心静脉压。对照组（n = 10）和抗体治疗组动物（n = 5）的心输出量无显著变化。在1小时内给予0.1 mg kg-1 R2内毒素的动物（n = 5）的心输出量显著增加了65%（P < 0.01）。在同时给予抗体和内毒素的大鼠中（n = 5），这种增加被消除。这项研究提供了证据，表明针对核心LPS的单克隆抗体可消除内毒素输注诱导的高动力状态。
