Raymaekers G, Aerssens J, Van den Eynde R, Peeters J, Geusens P, Devos P, Dequeker J
Arthritis and Metabolic Bone Disease Research Unit, K.U. Leuven, University Hospital Pellenberg, Belgium.
Calcif Tissue Int. 1992 Oct;51(4):269-75. doi: 10.1007/BF00334486.
The relation between bone mineralization and osteocalcin content was investigated in iliac crest cortical bone obtained at necropsy in young females and in two groups of elderly women with and without osteoarthritis of the hands evaluated by X-ray. Using density fractionation technique, the bone was separated into fractions of increasing density from 1.72 to 2.30 g/ml. The mineralization profile revealed a significant shift to higher densities in the osteoarthritis cases compared with young adults (P less than 0.005) and age-sex-matched controls (P less than 0.001). The ash, calcium, and phosphorus content of the bone increased with increasing density of the fractions whereas collagen content, measured as hydroxyproline, decreased. The osteocalcin concentration of each fraction was determined in the supernatants obtained after EDTA-extraction in the presence of protease inhibitors. In the young control and osteoarthritis group, the osteocalcin content in the lowest density fractions was higher compared with the older non-osteoarthritic group. Osteocalcin content of the high density fractions, representing highly mineralized osteons, was the same in the three groups studied. These findings support the hypothesis that quality differences in bone may explain the inverse relationship between osteoarthritis and osteoporosis.
在年轻女性尸检时获取的髂嵴皮质骨以及两组经X线评估有或无手部骨关节炎的老年女性中，研究了骨矿化与骨钙素含量之间的关系。采用密度分级技术，将骨分离成密度从1.72至2.30 g/ml逐渐增加的级分。矿化曲线显示，与年轻成年人（P<0.005）和年龄性别匹配的对照组（P<0.001）相比，骨关节炎病例的骨向更高密度有显著偏移。骨的灰分、钙和磷含量随级分密度增加而增加，而以羟脯氨酸衡量的胶原蛋白含量则下降。在存在蛋白酶抑制剂的情况下，通过EDTA提取后获得的上清液中测定每个级分的骨钙素浓度。在年轻对照组和骨关节炎组中，最低密度级分中的骨钙素含量高于老年非骨关节炎组。在研究的三组中，代表高度矿化骨单位的高密度级分的骨钙素含量相同。这些发现支持以下假设：骨质量差异可能解释骨关节炎与骨质疏松症之间的负相关关系。
