Notoya K, Tsukuda R, Yoshida K, Taketomi S
Pharmaceutical Research Laboratories II, Takeda Chemical Industries, Ltd., Osaka, Japan.
Calcif Tissue Int. 1992;51 Suppl 1:S16-20. doi: 10.1007/BF02180244.
The effects of ipriflavone (IP) (10(-5) M) on bone formation were studied in stromal cells from the femoral bone marrow of young adult rats cultured for 21 days in the presence of beta-glycerophosphate and dexamethasone. Stereoscopic microscopy showed nodule formation after 14 days of culturing, and both the number and the size of the nodules increased with time. The alizarin-red-stained calcified area in the nodules in the IP group was nearly 4 times as large as that in the control after 21 days. Light and electron microscopy revealed the presence of many osteoblast-like cells with developed rough endoplasmic reticulum and Golgi apparatus in the nodules in the control group after 14 days, and a collagenous fibril network was seen among the cells. After 21 days, calcification of the dense collagenous fibril network and bone matrix-like tissue were observed in many nodules, resulting in the formation of bone-like tissue containing osteocyte-like cells. In the IP group, the collagenous fibril network area in the nodules was greater than that in the control after 14 days, and a further increase in both the dense collagenous fibril network area and calcified bone-like tissue area was observed after 21 days. These findings indicate that IP stimulates bone-like tissue formation in the rat bone marrow stromal cell culture, suggesting that the promotion of collagen production by osteoblasts is involved in the stimulation of bone-like tissue formation by IP.
研究了淫羊藿黄酮（IP）（10⁻⁵ M）对年轻成年大鼠股骨骨髓基质细胞骨形成的影响，这些细胞在β-甘油磷酸酯和地塞米松存在的情况下培养21天。立体显微镜显示培养14天后有结节形成，结节的数量和大小均随时间增加。21天后，IP组结节中茜素红染色的钙化面积几乎是对照组的4倍。光镜和电镜显示，对照组14天后结节中有许多成骨细胞样细胞，其粗面内质网和高尔基体发达，细胞间可见胶原纤维网络。21天后，许多结节中观察到致密胶原纤维网络和骨基质样组织的钙化，形成了含有骨细胞样细胞的骨样组织。在IP组，14天后结节中的胶原纤维网络面积大于对照组，21天后致密胶原纤维网络面积和钙化骨样组织面积均进一步增加。这些结果表明，IP刺激大鼠骨髓基质细胞培养中的骨样组织形成，提示成骨细胞胶原生成的促进作用参与了IP对骨样组织形成的刺激。
