Smithson G, Wolcott R M, Chervenak R
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
Cell Immunol. 1992 Nov;145(1):30-42. doi: 10.1016/0008-8749(92)90310-l.
We have utilized several clonal cell lines, derived from the murine lymphoma ASL1w, to investigate the early events in NK-mediated lysis. The studies described here examine the relationship between NK recognition, NK cell:tumor cell conjugate formation, and NK-mediated lysis. The AW4F and AW4D tumor lines were susceptible to NK-mediated lysis and efficiently inhibited NK recognition in competitive inhibition assays, whereas the AW5J tumor, which is relatively resistant to NK-mediated lysis, did not. In contrast, the AW5E tumor was NK resistant but inhibited NK recognition almost as well as the NK-sensitive tumors, suggesting that it was deficient in a postbinding event required for NK-mediated lysis. These findings demonstrate a correlation, with one exception, between the susceptibility of the ASL1w-derived tumor lines to NK-mediated lysis and their ability to inhibit NK recognition. In contrast, there was no apparent correlation between tight conjugate formation, as assessed in three independent target binding assays, and the susceptibility of these tumors to NK-mediated lysis, showing that tight conjugate formation is not required for either efficient NK recognition or lysis.
我们利用了几种源自小鼠淋巴瘤ASL1w的克隆细胞系，来研究自然杀伤细胞（NK）介导的细胞裂解早期事件。此处描述的研究考察了NK识别、NK细胞与肿瘤细胞共轭体形成以及NK介导的细胞裂解之间的关系。AW4F和AW4D肿瘤细胞系易被NK介导的细胞裂解作用所影响，并且在竞争性抑制试验中能有效抑制NK识别，而对NK介导的细胞裂解相对抗性的AW5J肿瘤细胞系则不然。相比之下，AW5E肿瘤细胞系对NK具有抗性，但几乎与对NK敏感的肿瘤细胞系一样能抑制NK识别，这表明它在NK介导的细胞裂解所需的结合后事件中存在缺陷。这些发现证明，除了一个例外，源自ASL1w的肿瘤细胞系对NK介导的细胞裂解的敏感性与其抑制NK识别的能力之间存在相关性。相比之下，在三项独立的靶细胞结合试验中评估的紧密共轭体形成与这些肿瘤对NK介导的细胞裂解的敏感性之间没有明显的相关性，这表明高效的NK识别或裂解都不需要紧密共轭体形成。
