Chang I K, Tajima A, Yasuda Y, Chikamune T, Ohno T
RIKEN Cell Bank, Institute of Physical and Chemical Research, Ibaraki, Japan.
Cell Biol Int Rep. 1992 Sep;16(9):853-7. doi: 10.1016/s0309-1651(06)80165-7.
A simple one step centrifugation method was developed for purification of primordial germ cells (PGCs) of chick embryos. PGCs, constituting less than 0.1% of the total blood cells of stage 13-14 embryos that contained a microliter amount of blood, were concentrated at the interface of a 6.3% (w/v) and 14.4% (w/v) Ficoll bilayer by centrifugation at 800 x g for 30 min. the purity of these PGCs was 86%, which was 22 times that obtained previously.
开发了一种简单的一步离心法来纯化鸡胚原始生殖细胞（PGCs）。在含有微升量血液的13 - 14期胚胎中，PGCs占总血细胞的比例不到0.1%，通过在800×g下离心30分钟，这些PGCs在6.3%（w/v）和14.4%（w/v）的菲可双相层界面处富集。这些PGCs的纯度为86%，是之前所获纯度的22倍。
