COHEN G H, JOHNSTONE D B
J Bacteriol. 1964 Dec;88(6):1695-9. doi: 10.1128/jb.88.6.1695-1699.1964.
Cohen, Gary H. (University of Vermont, Burlington), and Donald B. Johnstone. Capsular polysaccharide of Azotobacter agilis. J. Bacteriol. 88:1695-1699. 1964.-Capsular polysaccharide from Azotobacter agilis strain 132 was recovered from washed cells by alkaline digestion. The polysaccharide was purified by centrifugation, repeated alcohol precipitation, Sevag deproteinization, and treatment with ribonuclease and charcoal-cellulose. Methods of isolation and purification appeared to provide a polymer showing no evidence of heterogeneity when examined by chemical and physical methods. Colorimetric, paper chromatographic, and enzymatic analyses on both intact and acid-hydrolyzed polysaccharide indicated that the polymer contained galactose and rhamnose at a molar ratio of approximately 1.0:0.7. A sialic acid-like component was also present in the polysaccharide. The study shows significant differences in the chemical composition of the extra-cellular polysaccharide of A. agilis and that of A. vinelandii. This adds further biochemical evidence for the right of these species to independent status.
科恩，加里·H.（佛蒙特大学，伯灵顿）和唐纳德·B.约翰斯通。敏捷固氮菌的荚膜多糖。《细菌学杂志》88:1695 - 1699。1964年。- 通过碱性消化从洗涤过的细胞中回收了敏捷固氮菌132菌株的荚膜多糖。通过离心、反复乙醇沉淀、塞瓦格脱蛋白以及用核糖核酸酶和活性炭 - 纤维素处理对多糖进行了纯化。分离和纯化方法似乎提供了一种在化学和物理方法检测时没有异质性迹象的聚合物。对完整和酸水解多糖的比色、纸色谱和酶分析表明，该聚合物含有半乳糖和鼠李糖，摩尔比约为1.0:0.7。多糖中还存在一种类似唾液酸的成分。该研究表明敏捷固氮菌和褐球固氮菌细胞外多糖的化学组成存在显著差异。这为这些物种具有独立地位的观点增加了进一步的生化证据。
