CHEN R F, BOWMAN R L
Science. 1965 Feb 12;147(3659):729-32. doi: 10.1126/science.147.3659.729.
Ultraviolet-polarizing filters with large apertures facilitate the use of spectrophotofluorometers for measuring fluorescence polarization. Such filters (i) allow the use of polarized exciting light even in the ultraviolet region, (ii) make it possible to correct for grating anomalies which influence the observed polarization, and (iii) because of good transmission properties allow the entrance of sufficient light so that small slits and dilute solutions can be used. Under optimum conditions, polarization can be measured with a precision of +/-0.002. Results obtained are in excellent agreement with values cited elsewhere and indicate that standard spectrophotofluorometers can easily be adapted for accurate measurement of polarization.
大孔径紫外偏振滤光片便于使用分光光度计测量荧光偏振。这种滤光片：(i) 即使在紫外区域也能使用偏振激发光；(ii) 能够校正影响观测偏振的光栅异常；(iii) 由于具有良好的透射特性，能使足够的光进入，从而可以使用小狭缝和稀溶液。在最佳条件下，偏振测量精度可达±0.002。所得结果与其他地方引用的值非常吻合，表明标准分光光度计可轻松适配用于精确测量偏振。
