电离辐射：辐照介质对合成过程的影响。

IONIZING RADIATION: EFFECT OF IRRADIATED MEDIUM ON SYNTHETIC PROCESSES.

作者信息

POLLARD E C, EBERT M J, MILLER C, KOLACZ K, BARONE T F

出版信息

Science. 1965 Feb 26;147(3661):1045-7. doi: 10.1126/science.147.3661.1045.

DOI:10.1126/science.147.3661.1045

Abstract

The incorporation of uracil-C(14) into macromolecules in Escherichia coli cells is decreased by doses of ionizing radiation when the cells are in very dilute suspension. The decrease results from an action of irradiated medium on the cells, and a similar reaction is observed during the incorporation of thymine (indication of DNA synthesis) and of proline and valine (indicative of protein synthesis). Irradiated medium reduces the formation of beta-galactosidase but does not cause the degradation of DNA.

摘要

当大肠杆菌细胞处于非常稀的悬浮液中时，电离辐射剂量会降低尿嘧啶-C(14)掺入这些细胞大分子中的量。这种降低是由辐照培养基对细胞的作用导致的，并且在胸腺嘧啶掺入（DNA合成的指标）以及脯氨酸和缬氨酸掺入（蛋白质合成的指标）过程中观察到类似反应。辐照培养基会减少β-半乳糖苷酶的形成，但不会导致DNA降解。