Fulcher S F, Foulks G N, Wilkerson M, Cobo L M, Jaffe G J, Hatchell D L
Duke University Eye Center, Durham, North Carolina.
Cornea. 1992 Sep;11(5):413-7. doi: 10.1097/00003226-199209000-00010.
We examined the effects of the immunotoxin 454A12-rRa on proliferating and confluent human corneal epithelium (HCE) in vitro. Proliferating HCE was sensitive to 454A12-rRA in a dose-dependent fashion. At immunotoxin concentrations of 1,000 ng/ml for 7 days we observed an 86% reduction in cell counts. Confluent HCE was not sensitive to 454A12-rRA at equivalent concentrations of immunotoxin. These data confirm previous observations regarding selective sensitivity of proliferating ocular tissue to immunotoxin, but suggest that HCE is less sensitive to 454A12-rRA than other ocular cell types.
