Saint-Macary M, Guérin B, Foucher B
Biochimie. 1977;59(5-6):509-15. doi: 10.1016/s0300-9084(77)80056-4.
The inhibitory effect of oligomycin was investigated in intact mitochondria through oxidative phosphorylation and uncoupler induced ATPase activity. Results show that oligomycin inhibition curves can be either sigmoidal or hyperbolic depending on experimental conditions and chiefly on the metabolic state of mitochondria with regard to the distribution of mitochondrial endogenous adenine-nucleotides. Active respiration and uncoupler-induced ATPse activity produce sigmoidal titration curves for a high initial ATP : ADP ratio and hyperbolic curves for a low ATP : ADP ratio. Time-dependent inhibitions are observed for the two reactions. The maximal inhibitory action for low concentrations of the inhibitor is delayed by the initial presence of ATP or the possibility of generating from inorganic phosphate before adding oligomycin. Results presented here show that the initial adenine-nucleotide distribution is important for oligomycin sensitivity of energy-linked reactions. Although a limited conformational change of the oligomycin-sensitivity to the inhibitor, it is more likely that a gross structural change of the inner membrane induced by adenine-nucleotides modifies membrane permeability to oligomycin.
通过氧化磷酸化和解偶联剂诱导的ATP酶活性，在完整的线粒体中研究了寡霉素的抑制作用。结果表明，寡霉素抑制曲线可以是S形或双曲线形，这取决于实验条件，主要取决于线粒体关于内源性腺嘌呤核苷酸分布的代谢状态。活跃呼吸和解偶联剂诱导的ATP酶活性，对于高初始ATP:ADP比值产生S形滴定曲线，对于低ATP:ADP比值产生双曲线形曲线。在这两个反应中观察到时间依赖性抑制。对于低浓度抑制剂的最大抑制作用，会被ATP的初始存在或在添加寡霉素之前从无机磷酸盐生成ATP的可能性所延迟。此处给出的结果表明，初始腺嘌呤核苷酸分布对于能量偶联反应的寡霉素敏感性很重要。尽管寡霉素对抑制剂的敏感性存在有限的构象变化，但更有可能的是，腺嘌呤核苷酸诱导的内膜总体结构变化改变了膜对寡霉素的通透性。
