BEKIERKUNST A, BERRY L J
J Bacteriol. 1965 Jan;89(1):205-11. doi: 10.1128/jb.89.1.205-211.1965.
Bekierkunst, Adam (Bryn Mawr College, Bryn Mawr, Pa.), and L. Joe Berry. Cytotoxic effect of mycobacteria on Ehrlich ascites cells. J. Bacteriol. 89:205-211. 1965.-Mycobacterium tuberculosis, strains H(37)Rv, H(37)Ra, and BCG, release into a medium within 3 to 5 hr a substance that is toxic to Ehrlich ascites cells as evidenced by a diminished packed cell volume after 5 to 7 days of growth in the peritoneal cavity of mice. Cell-free extracts of the above strains of mycobacteria display the same type of cytotoxic effect, the strongest action being that of the virulent strain, H(37)Rv. The nicotinamide adenine dinucleotidase activity of surviving tumor cells was three to four times greater after treatment with the mycobacterial extracts than that of control cells. The cycotoxicity is probably due to cord factor, because 10 mug of this substance injected intraperitoneally into mice bearing Ehrlich ascites cells inhibited the growth of the cells to 50% of that of control cells. An increase in nicotinamide adenine dinucleotidase activity was found in surviving tumor cells after cord factor treatment.
贝基尔昆斯特，亚当（宾夕法尼亚州布林莫尔学院，布林莫尔），以及L.乔·贝里。分枝杆菌对艾氏腹水癌细胞的细胞毒性作用。《细菌学杂志》89:205 - 211。1965年。——结核分枝杆菌H(37)Rv、H(37)Ra菌株以及卡介苗，在3至5小时内会向培养基中释放一种对艾氏腹水癌细胞有毒的物质，这可通过在小鼠腹腔中生长5至7天后细胞压积体积减小得到证明。上述分枝杆菌菌株的无细胞提取物表现出相同类型的细胞毒性作用，其中毒性最强的是强毒株H(37)Rv。用分枝杆菌提取物处理后，存活肿瘤细胞的烟酰胺腺嘌呤二核苷酸酶活性比对照细胞高3至4倍。这种细胞毒性可能归因于索状因子，因为将10微克该物质腹腔注射到携带艾氏腹水癌细胞的小鼠体内，可使细胞生长抑制至对照细胞的50%。在用索状因子处理后，存活肿瘤细胞中发现烟酰胺腺嘌呤二核苷酸酶活性增加。
