Achike F I, Dai S, Ogle C W
Department of Pharmacology, Faculty of Medicine, University of Hong Kong.
Eur J Pharmacol. 1992 Sep 4;219(3):369-76. doi: 10.1016/0014-2999(92)90477-l.
Hearts from male Sprague-Dawley rats were perfused, by the Lagendorff method, with calcium-free Krebs solution (containing either adrenaline or a high level of potassium) at pH 7.48 (control), 7.26 (acidosis) or 7.69 (alkalosis). When the hearts stopped contracting, a dose of nifedipine or its vehicle was given before measuring the force of contraction, coronary perfusion pressure and heart rate in response to graded doses of calcium. The calcium-antagonising efficacy of nifedipine was reduced during acidosis in both adrenaline- and potassium-stimulated hearts, but the reduction was greater in the adrenaline-stimulated hearts. Alkalosis led to a small increase in the efficacy of nifedipine on adrenaline- and potassium-stimulated contractions.
采用Langendorff方法，用pH值为7.48（对照）、7.26（酸中毒）或7.69（碱中毒）的无钙Krebs溶液（含有肾上腺素或高浓度钾）灌注雄性Sprague-Dawley大鼠的心脏。当心脏停止收缩时，在测量对不同剂量钙的收缩力、冠状动脉灌注压和心率之前，给予一剂硝苯地平或其赋形剂。在酸中毒期间，肾上腺素刺激和钾刺激的心脏中，硝苯地平的钙拮抗效力均降低，但在肾上腺素刺激的心脏中降低幅度更大。碱中毒导致硝苯地平对肾上腺素刺激和钾刺激的收缩的效力略有增加。
