Yadid G, Youdim M B, Zinder O
Technion-Bruce Rappaport Faculty of Medicine, Department of Pharmacology, Haifa, Israel.
Eur J Pharmacol. 1992 Oct 20;221(2-3):389-91. doi: 10.1016/0014-2999(92)90729-n.
Isolated adrenal chromaffin cells were used as a model for the release of catecholamines from adrenergic nerve endings. In this study we used an HPLC technique to determine the effects of acetylcholine and glycine on norepinephrine and epinephrine release. The amount of catecholamine released in response to glycine was 22% less than that released by acetylcholine. However, while the norepinephrine-to-epinephrine ratio was 1.6 after stimulation with acetylcholine, it was 0.6 after stimulation with glycine. This suggests that glycine preferentially affects epinephrine secretion as compared to acetylcholine, which preferentially releases norepinephrine. This differential effect could be of physiological importance considering our recent demonstration of a functional high-affinity chloride-gated glycine receptor on adrenal chromaffin cells.
分离的肾上腺嗜铬细胞被用作去甲肾上腺素能神经末梢释放儿茶酚胺的模型。在本研究中，我们使用高效液相色谱技术来确定乙酰胆碱和甘氨酸对去甲肾上腺素和肾上腺素释放的影响。甘氨酸刺激后释放的儿茶酚胺量比乙酰胆碱刺激后释放的量少22%。然而，乙酰胆碱刺激后去甲肾上腺素与肾上腺素的比例为1.6，而甘氨酸刺激后该比例为0.6。这表明与优先释放去甲肾上腺素的乙酰胆碱相比，甘氨酸优先影响肾上腺素的分泌。考虑到我们最近在肾上腺嗜铬细胞上证明了一种功能性高亲和力氯离子门控甘氨酸受体，这种差异效应可能具有生理重要性。
