Ohashi K, Saji F, Kato M, Okabe M, Mimura T, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Fertil Steril. 1992 Oct;58(4):803-8.
To evaluate the acrosomal status of viable sperm by MH61-beads test and its clinical application.
Acrosomal status was evaluated using immunobeads coated with MH61 monoclonal antibody, which is specific for acrosome-reacted sperm. When viable sperm was coincubated with MH61-beads for an appropriate length of time, the formation of a sperm-bead complex was observed with a phase-contrast microscope, and the number of sperm binding to MH61-beads increased with the progression of the acrosome reaction. Using this agglutination, we developed the MH61-beads test to assess sperm function.
Department of Obstetrics and Gynecology, Osaka University Hospital.
Forty-three volunteers and 20 males in our in vitro fertilization (IVF) and embryo transfer program.
The results of MH61-beads test were compared with the percentage of acrosome-reacted sperm detected by Pisium satinum agglutinin staining on 30 volunteers, with the results of sperm penetration assay (SPA) in 43 volunteers, and with the outcome of IVF in 20 patients.
The results of MH61-beads test showed good reproducibility and correlated with the results of SPA using zona-free hamster eggs and IVF.
The MH61-beads test, the results of which reflect the acrosomal status of sperm, may provide useful information concerning the fertilizing ability of sperm.
通过MH61珠试验评估活精子的顶体状态及其临床应用。
使用包被有MH61单克隆抗体的免疫珠评估顶体状态，该抗体对顶体反应的精子具有特异性。当活精子与MH61珠共同孵育适当时间后，用相差显微镜观察精子-珠复合物的形成，并且随着顶体反应的进行，与MH61珠结合的精子数量增加。利用这种凝集反应，我们开发了MH61珠试验来评估精子功能。
大阪大学医院妇产科。
43名志愿者以及参与我们体外受精（IVF）和胚胎移植项目的20名男性。
将30名志愿者的MH61珠试验结果与通过豌豆凝集素染色检测的顶体反应精子百分比进行比较，将43名志愿者的精子穿透试验（SPA）结果与之比较，并将20名患者的IVF结果与之比较。
MH61珠试验结果显示出良好的重复性，并且与使用无透明带仓鼠卵的SPA结果以及IVF结果相关。
MH61珠试验结果反映精子的顶体状态，可能为精子受精能力提供有用信息。
