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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Haeffner E W, Strosznajder J B

Institut für Zell- und Tumorbiologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Int J Biochem. 1992 Sep;24(9):1481-5. doi: 10.1016/0020-711x(92)90075-c.

A rapid uptake and esterification of [14C]arachidonic acid during the first 4 hr of cultivation of ascites cells in serum-deprived medium was observed followed by a fast turnover of the fatty acid. 2. Labeling and turnover of esterified arachidonate in individual phospholipid classes was in the order: phosphatidylcholine (PC) greater than phosphatidylinositol (PI) much greater than phosphatidylinositol-4-phosphate (PIP) and -4,5-bisphosphate (PIP2) greater than phosphatidylethanolamine (PE) greater than PE-plasmalogens. 3. In cells stimulated with 1-oleoyl-2-acetyl-sn-glycerol a transient course of arachidonic acid incorporation into PC, PI, PIP and PIP2 was determined peaking 30 min after stimulation, indicating both esterification and release under these conditions. 4. The release of arachidonate was blocked by quinacrine which is a specific inhibitor of phospholipase A2.

在无血清培养基中培养腹水细胞的最初4小时内，观察到[14C]花生四烯酸的快速摄取和酯化，随后脂肪酸快速周转。2. 各个磷脂类中酯化花生四烯酸的标记和周转顺序为：磷脂酰胆碱（PC）＞磷脂酰肌醇（PI）＞＞磷脂酰肌醇-4-磷酸（PIP）和-4,5-二磷酸（PIP2）＞磷脂酰乙醇胺（PE）＞PE-缩醛磷脂。3. 在用1-油酰基-2-乙酰基-sn-甘油刺激的细胞中，测定了花生四烯酸掺入PC、PI、PIP和PIP2的短暂过程，在刺激后30分钟达到峰值，表明在这些条件下既有酯化又有释放。4. 花生四烯酸的释放被喹吖因阻断，喹吖因是磷脂酶A2的特异性抑制剂。

Haeffner E W, Strosznajder J B

Institut für Zell- und Tumorbiologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Int J Biochem. 1992 Sep;24(9):1481-5. doi: 10.1016/0020-711x(92)90075-c.

A rapid uptake and esterification of [14C]arachidonic acid during the first 4 hr of cultivation of ascites cells in serum-deprived medium was observed followed by a fast turnover of the fatty acid. 2. Labeling and turnover of esterified arachidonate in individual phospholipid classes was in the order: phosphatidylcholine (PC) greater than phosphatidylinositol (PI) much greater than phosphatidylinositol-4-phosphate (PIP) and -4,5-bisphosphate (PIP2) greater than phosphatidylethanolamine (PE) greater than PE-plasmalogens. 3. In cells stimulated with 1-oleoyl-2-acetyl-sn-glycerol a transient course of arachidonic acid incorporation into PC, PI, PIP and PIP2 was determined peaking 30 min after stimulation, indicating both esterification and release under these conditions. 4. The release of arachidonate was blocked by quinacrine which is a specific inhibitor of phospholipase A2.

在无血清培养基中培养腹水细胞的最初4小时内，观察到[14C]花生四烯酸的快速摄取和酯化，随后脂肪酸快速周转。2. 各个磷脂类中酯化花生四烯酸的标记和周转顺序为：磷脂酰胆碱（PC）＞磷脂酰肌醇（PI）＞＞磷脂酰肌醇-4-磷酸（PIP）和-4,5-二磷酸（PIP2）＞磷脂酰乙醇胺（PE）＞PE-缩醛磷脂。3. 在用1-油酰基-2-乙酰基-sn-甘油刺激的细胞中，测定了花生四烯酸掺入PC、PI、PIP和PIP2的短暂过程，在刺激后30分钟达到峰值，表明在这些条件下既有酯化又有释放。4. 花生四烯酸的释放被喹吖因阻断，喹吖因是磷脂酶A2的特异性抑制剂。