Charpin G, Chikh-Issa A R, Guignard H, Jourdan G, Dumas C, Pansu D, Descroix-Vagne M
INSERM U 45, Hôpital E. Herriot, Lyon, France.
Gastroenterology. 1992 Nov;103(5):1568-73. doi: 10.1016/0016-5085(92)91179-8.
Sorbin is a newly isolated intestinal peptide that has been purified because of its ability to induce water absorption. The effects that sorbin and some synthetic peptides corresponding to its C-terminal sequence have on duodenal absorption of water, chloride, and sodium were studied in comparison with the effects of vasoactive intestinal peptide (VIP), [D-Ala,Met]-enkephalinamide (DAMA), and angiotensin II. The technique of an in situ ligated duodenal loop in the rat was used for all peptides. Under the experimental conditions used, a low basal secretion of water, chloride, and sodium was obtained; VIP induced an increase of the secretion, whereas DAMA induced an absorption, both in the nanomolar dose range. Angiotensin II and sorbin induced an absorption in the picomolar dose range. The most effective doses of sorbin peptides but not of angiotensin induced the lowest final concentrations of Na+ and Cl- obtainable without inducing secondary water secretion. All synthetic peptides containing the C-terminal heptapeptide of sorbin were active in the picomolar dose range. Contrary to angiotensin, they had no effect on blood pressure.
索宾是一种新分离出的肠肽，因其具有诱导水吸收的能力而被纯化。与血管活性肠肽（VIP）、[D-丙氨酸，甲硫氨酸] - 脑啡肽酰胺（DAMA）和血管紧张素II的作用相比，研究了索宾及其一些对应于其C末端序列的合成肽对十二指肠水、氯和钠吸收的影响。所有肽均采用大鼠原位结扎十二指肠袢技术进行研究。在所使用的实验条件下，获得了较低的水、氯和钠基础分泌量；在纳摩尔剂量范围内，VIP诱导分泌增加，而DAMA诱导吸收。血管紧张素II和索宾在皮摩尔剂量范围内诱导吸收。索宾肽而非血管紧张素的最有效剂量在不诱导继发性水分泌的情况下可获得最低的最终Na +和Cl-浓度。所有含有索宾C末端七肽的合成肽在皮摩尔剂量范围内均具有活性。与血管紧张素相反，它们对血压没有影响。
