Marquet F, Botella A, Bueno L, Pansu D, Descroix-Vagne M
INSERM, Unité 45, Hôpital E Herriot, Lyon, France.
Peptides. 1998;19(8):1417-23. doi: 10.1016/s0196-9781(98)00082-5.
The effect of synthetic sorbin derivatives was determined on cholera toxin-stimulated jejunal secretion in anesthetized rats in vivo, using both perfused and ligated loop. An inhibitory effect on water secretion induced by cholera toxin was shown with C-terminal sorbin peptides: C20 (YEPGKSSILQHERPVTKPQA-amide), C10 and Dala 7 heptapeptide-amide of sorbin, given by subcutaneous (SC) or intraduodenal administration. When perfused intravenously, C20-sorbin inhibited the cholera-induced stimulation of net flux of water, Na+ and K+, in the jejunum and at the same time the net flux of water and Cl- in the duodenum, which was not in contact with the toxin. 5-hydroxytryptamine was not significantly changed in plasma or fluid. Prostaglandin E2 release in jejunal as well as duodenal fluid was significantly stimulated by cholera toxin, but was not significantly different from basal value after C20 administration.
利用灌注和结扎肠袢的方法，在体内对麻醉大鼠进行实验，以确定合成山梨醇衍生物对霍乱毒素刺激的空肠分泌的影响。C末端山梨醇肽：C20（YEPGKSSILQHERPVTKPQA-酰胺）、C10和山梨醇的Dala 7七肽酰胺，通过皮下（SC）或十二指肠内给药，显示出对霍乱毒素诱导的水分泌有抑制作用。当静脉灌注时，C20-山梨醇抑制了霍乱诱导的空肠中水、Na+和K+的净通量刺激，同时也抑制了未接触毒素的十二指肠中水和Cl-的净通量。血浆或液体中的5-羟色胺没有显著变化。霍乱毒素显著刺激空肠和十二指肠液中前列腺素E2的释放，但在给予C20后与基础值没有显著差异。
