GAINES S, CURRIE J A, TULLY J G
J Bacteriol. 1965 Mar;89(3):776-81. doi: 10.1128/jb.89.3.776-781.1965.
Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by means of active immunization tests with tissue extracts. Mice were injected intraperitoneally with purified Vi antigen or Vi-containing bacilli. At appropriate intervals, animals were killed, and saline extracts of their tissues were prepared. Mice were immunized with these extracts and challenged 6 days later with 10 ld(50) of Salmonella typhosa Ty2. Protection was afforded by tissue extracts from Vi-injected mice, but not by normal tissue extracts. That the immunizing capacity of tissue extracts from Vi-injected mice was attributable to Vi antigen was affirmed by the demonstration that these extracts stimulated the production of Vi antibody in mice, coated erythrocytes for agglutination by Vi antiserum, and inhibited agglutination of Vi-sensitized red blood cells by known Vi antisera. Vi antigen could be detected in the liver and spleen of mice injected with as little as 1 mug. In mice given 150 mug, the antigen was still present in liver tissue 231 days later.
盖恩斯，西德尼（华盛顿特区沃尔特·里德陆军研究所）、朱利叶斯·A·柯里和约瑟夫·G·塔利。主动免疫小鼠组织中Vi抗原的检测与持续存在。《细菌学杂志》89:776 - 781。1965年。——通过用组织提取物进行主动免疫试验来确定Vi抗原在小鼠组织中的存在、分布和持续存在情况。给小鼠腹腔注射纯化的Vi抗原或含Vi的杆菌。在适当的间隔时间，处死动物，并制备其组织的盐水提取物。用这些提取物对小鼠进行免疫，6天后用10个半数致死量（50）的伤寒沙门氏菌Ty2进行攻击。注射Vi的小鼠的组织提取物提供了保护作用，而正常组织提取物则没有。通过证明这些提取物能刺激小鼠产生Vi抗体、使红细胞被Vi抗血清包被以进行凝集以及抑制已知Vi抗血清对Vi致敏红细胞的凝集，证实了注射Vi的小鼠的组织提取物的免疫能力归因于Vi抗原。在注射低至1微克的小鼠的肝脏和脾脏中可检测到Vi抗原。在给予150微克的小鼠中，231天后肝脏组织中仍存在该抗原。
