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水螅中的细胞增殖:一种放射自显影方法。

CELL PROLIFERATION IN HYDRA: AN AUTORADIOGRAPHIC APPROACH.

作者信息

CAMPBELL R D

出版信息

Science. 1965 May 28;148(3674):1231-2. doi: 10.1126/science.148.3674.1231.

Abstract

Hydra cells take up substantial amounts of tritiated thymidine if the label is injected into an animal's gastric cavity shortly after feeding. Autoradiographic analysis shows that the isotope becomes incorporated into cell nuclei. The distribution of labeled nuclei probably represents the pattern of cell proliferation and is similar to the distribution of mitotic figures in histological preparations. The data show that cell proliferation is distributed along the body column and that there is no localized growth zone.

摘要

如果在喂食后不久将标记物注入动物的胃腔,水螅细胞会摄取大量的氚标记胸腺嘧啶核苷。放射自显影分析表明,该同位素被整合到细胞核中。标记细胞核的分布可能代表细胞增殖模式,并且与组织学切片中有丝分裂图像的分布相似。数据表明,细胞增殖沿体柱分布,且不存在局部生长区。

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