Chandra R S, Dandekar U P, Karnard D, Tilve G H, Kshirsagar N A
Department of Pharmacology, Seth GS Medical College & KEM Hospital, Bombay.
Indian J Med Res. 1992 Apr;96:109-11.
An in vitro study of the effect of immunoglobulins (mainly IgG) on opsonophagocytic activity of polymorphs was carried out in 17 tracheostomised patients admitted in medical intensive care unit of our hospital. The opsonic and phagocytic activities were tested against Staphylococcus aureus by modified polymorphonuclear leucocyte overlay method; and serum IgG and serum IgM levels were estimated by single radial immunodiffusion technique. As compared to healthy volunteers, opsonophagocytic activity was significantly lower in tracheostomised patients. However, this activity improved markedly after immunoglobulin supplementation (P less than 0.01). The same degree of enhancement was also observed in normal controls.
在我院医学重症监护病房收治的17例气管切开患者中，开展了一项关于免疫球蛋白（主要为IgG）对多形核白细胞调理吞噬活性影响的体外研究。采用改良的多形核白细胞覆盖法检测针对金黄色葡萄球菌的调理和吞噬活性；通过单向放射免疫扩散技术测定血清IgG和血清IgM水平。与健康志愿者相比，气管切开患者的调理吞噬活性显著降低。然而，补充免疫球蛋白后该活性显著改善（P<0.01）。在正常对照中也观察到了相同程度的增强。
