Sarkar C, Dinda A K, Roy S, Kochupillai N, Kharbanda K, Tandon P N
Department of Pathology, All India Institute of Medical Sciences, New Delhi.
Indian J Med Res. 1992 Aug;96:209-14.
An ultrastructural study was done on 15 mixed growth hormone (GH) and prolactin (PRL)-secreting pituitary adenomas surgically removed from acromegalic patients with hyper-prolactinaemia, in order to see whether the 2 hormones were present in the same cell or in different cells. Double labelling immunogold technique was used for simultaneous ultrastructural localization of GH and PRL. It was found that each neoplastic cell in these 15 tumours (30 to 50 cells were studied in each case) contained 4 populations of granules viz., (i) granules positive for only GH; (ii) granules positive for only PRL; (iii) granules positive for both GH and PRL; and (iv) granules negative for both GH and PRL (unlabelled). Though the relative percentage of these 4 types of granules varied from cell to cell even within the same tumour, the major population (49.9 to 96%) was constituted by the mixed granules showing labelling for both GH and PRL. Almost all the cells examined from each tumour appeared to be mammosomatotrophs. Thus, the study indicated that mammosomatotroph adenomas are perhaps more common among mixed GH and PRL--secreting pituitary adenomas than previously believed. It could be important to recognize these tumours from the therapeutic point of view.
对15例手术切除的生长激素（GH）和催乳素（PRL）混合分泌型垂体腺瘤进行了超微结构研究，这些腺瘤来自患有高催乳素血症的肢端肥大症患者，目的是观察这两种激素是存在于同一细胞还是不同细胞中。采用双标记免疫金技术对GH和PRL进行超微结构同步定位。结果发现，这15个肿瘤中的每个肿瘤细胞（每个病例研究30至50个细胞）都含有4种颗粒群，即：（i）仅对GH呈阳性的颗粒；（ii）仅对PRL呈阳性的颗粒；（iii）对GH和PRL均呈阳性的颗粒；以及（iv）对GH和PRL均呈阴性的颗粒（未标记）。尽管这4种类型颗粒的相对百分比在同一肿瘤内的不同细胞之间也有所不同，但主要群体（49.9%至96%）是同时显示GH和PRL标记的混合颗粒。从每个肿瘤中检查的几乎所有细胞似乎都是乳腺生长激素细胞。因此，该研究表明，在生长激素和催乳素混合分泌型垂体腺瘤中，乳腺生长激素细胞腺瘤可能比以前认为的更为常见。从治疗角度认识这些肿瘤可能很重要。
