从艾氏腹水癌微粒体中分离和部分纯化质膜片段
SEPARATION AND PARTIAL PURIFICATION OF PLASMA-MEMBRANE FRAGMENTS FROM EHRLICH ASCITES CARCINOMA MICROSOMES.
作者信息
KAMAT V B, WALLACH D F
出版信息
Science. 1965 Jun 4;148(3675):1343-5. doi: 10.1126/science.148.3675.1343.
Abstract
Membrane vesicles arising from the surface of Ehrlich ascites carcinoma cells were separated from the bulk of other microsomal components in Ficoll density gradients. Initial separation depends on the differential action of magnesium ion on various microsomal components. Immunologic and enzymic markers were used to monitor separation and purification steps.
摘要
从艾氏腹水癌细胞表面产生的膜泡在Ficoll密度梯度中与大部分其他微粒体成分分离。初始分离取决于镁离子对各种微粒体成分的差异作用。使用免疫和酶标记物来监测分离和纯化步骤。
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