汞对近交系棕色挪威大鼠和刘易斯大鼠腹膜白细胞（多形核白细胞和巨噬细胞）的体外作用。

The in vitro effects of mercury on peritoneal leukocytes (PMN and macrophages) from inbred brown Norway and Lewis rats.

作者信息

Contrino J, Kosuda L L, Marucha P, Kreutzer D L, Bigazzi P E

机构信息

Department of Pathology, University of Connecticut Health Center, Farmington 06032.

出版信息

Int J Immunopharmacol. 1992 Aug;14(6):1051-9. doi: 10.1016/0192-0561(92)90150-j.

DOI:10.1016/0192-0561(92)90150-j

PMID:1428360

Abstract

The present paper demonstrates that HgCl2 can affect rat peritoneal polymorphonuclear leukocyte (PMN) and macrophage (M phi) functions in vitro. In addition, we have noticed that these effects of mercury vary according to the rat strain: for example, HgCl2 stimulates H2O2 release from Lewis (LEW) but not Brown Norway (BN) PMN. Similarly, LEW M phi produce high levels of H2O2 when exposed to HgCl2 in vitro, whereas BN M phi do not. Finally, mercury inhibits erythrophagocytosis of both LEW and BN "resident" peritoneal M phi. Preliminary experiments using M phi from other rat strains have also shown that MAXX M phi are stimulated by HgCl2 to release H2O2 in vitro, whereas Yoshida M phi are inhibited. Differences in lymphocyte responses (e.g. delayed-type hypersensitivity reactions and mitogen stimulation) between rats of various strains are well known. To these examples one may now add variations in PMN and M phi responses to mercury and possibly other metals. Our results suggest that caution should be exercised in interpreting the outcome of immunotoxicity studies in experimental animals. In particular, outbred rats may not provide appropriate models, that might be better obtained by comparative investigations of rats from various inbred strains.

摘要

本文证明，氯化汞在体外可影响大鼠腹膜多形核白细胞（PMN）和巨噬细胞（M phi）的功能。此外，我们还注意到汞的这些作用因大鼠品系而异：例如，氯化汞可刺激Lewis（LEW）大鼠的PMN释放过氧化氢，但对Brown Norway（BN）大鼠的PMN则无此作用。同样，LEW大鼠的M phi在体外暴露于氯化汞时会产生高水平的过氧化氢，而BN大鼠的M phi则不会。最后，汞抑制LEW和BN“驻留”腹膜M phi的红细胞吞噬作用。使用其他大鼠品系的M phi进行的初步实验也表明，MAXX大鼠的M phi在体外受氯化汞刺激可释放过氧化氢，而Yoshida大鼠的M phi则受到抑制。不同品系大鼠之间淋巴细胞反应（如迟发型超敏反应和丝裂原刺激）的差异是众所周知的。现在，在PMN和M phi对汞以及可能对其他金属的反应变化方面也可加入这些例子。我们的结果表明，在解释实验动物免疫毒性研究的结果时应谨慎。特别是，远交系大鼠可能无法提供合适的模型，通过对各种近交系大鼠进行比较研究可能会更好地获得此类模型。