重氮酞菁作为精细结构细胞化学试剂
DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY.
作者信息
TICE L W, BARRNETT R J
出版信息
J Cell Biol. 1965 Apr;25(1):23-41. doi: 10.1083/jcb.25.1.23.
Abstract
This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density increases thus produced were compared in the electron microscope with those produced by staining similarly fixed material with the phthalocyanin dye, Alcian Blue. Finally, one of the diazotates was used as a capture reagent for the demonstration of the sites of acid phosphatase activity with the electron microscope.
摘要
本文报道了14种含有镁、铜或铅作为螯合金属的重氮酞菁的合成。为了评估这些化合物在精细结构细胞化学中的实用性，测试了它们与萘酚的相对偶联率以及所得偶氮染料的溶解度。将其中三种重氮酸盐与醛固定材料中的组织蛋白反应，并在电子显微镜下将由此产生的密度增加与用酞菁染料阿尔辛蓝对类似固定材料染色所产生的密度增加进行比较。最后，将其中一种重氮酸盐用作捕获试剂，用电子显微镜显示酸性磷酸酶活性位点。
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1
Preferential staining of nucleic acid-containing structures for electron microscopy.
J Biophys Biochem Cytol. 1961 Nov;11(2):273-96. doi: 10.1083/jcb.11.2.273.
2
A diazo coupling method for the electron microscopic localization of cholinesterase.
J Biophys Biochem Cytol. 1959 Dec;6(3):399-406. doi: 10.1083/jcb.6.3.399.
3
Methods for the use of indium as an electron stain for nucleic acids.
J Biophys Biochem Cytol. 1961 Nov;11(2):257-72. doi: 10.1083/jcb.11.2.257.
4
Stability of the iodine-carbon bond of radioactive iodoalbumin in the 50 kv electron beam.
J Cell Biol. 1962 Nov;15(2):395. doi: 10.1083/jcb.15.2.395.
5
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.
J Cell Biol. 1963 Apr;17(1):19-58. doi: 10.1083/jcb.17.1.19.
6
New diazonium components as coupling agents in the demonstration of phosphatases.
J Histochem Cytochem. 1961 May;9:301-3. doi: 10.1177/9.3.301.
7
Electron stains. II: Electron microscopic studies on the visibility of stained DNA molecules.
J Mol Biol. 1961 Dec;3:717-26. doi: 10.1016/s0022-2836(61)80076-4.
8
A correlative study of the histochemical properties of rodent acid mucopolysaccharides.
J Histochem Cytochem. 1960 Jan;8:18-35. doi: 10.1177/8.1.18.
9
A new technique for the simultaneous demonstration of lipid and acid polysaccharides on the same tissue section.
J Histochem Cytochem. 1959 Mar;7(2):101-6. doi: 10.1177/7.2.101.
10
Histochemical demonstration of erythrocyte esterases.
Proc Soc Exp Biol Med. 1959 May;101(1):90-3. doi: 10.3181/00379727-101-24842.
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