嗜锇试剂：用于光学显微镜和电子显微镜的新细胞化学原理

OSMIOPHILIC REAGENTS: NEW CYTOCHEMICAL PRINCIPLE FOR LIGHT AND ELECTRON MICROSCOPY.

作者信息

HANKER J S, SEAMAN A R, WEISS L P, UENO H, BERGMAN R A, SELIGMAN A M

出版信息

Science. 1964 Nov 20;146(3647):1039-43. doi: 10.1126/science.146.3647.1039.

DOI:10.1126/science.146.3647.1039

PMID:14202455

Abstract

Established histochemical procedures have been altered by utilizing reagents which contain a group capable of reacting selectively with OsO(4). This permits the cytochemical demonstration of a particular enzyme or functional group of a macromolecule with the light and electron microscope. The osmiophilic groups used thus far are mercapto, thiocarbamyl, and diazothioether. The "osmium black" produced at the site of reaction is electron opaque, is insoluble in lipids, and is not altered when the issue is embedded in acrylic and epoxy resins. Its fine amorphous character enables it to be maintained precisely in the electron beam.

摘要

通过使用含有能够与四氧化锇选择性反应的基团的试剂，已对既定的组织化学程序进行了改进。这使得在光学显微镜和电子显微镜下能够对大分子的特定酶或官能团进行细胞化学显示。迄今为止所使用的嗜锇基团有巯基、硫代氨基甲酰基和重氮硫醚。在反应部位产生的“锇黑”在电子显微镜下是不透明的，不溶于脂质，并且当组织包埋在丙烯酸树脂和环氧树脂中时不会发生变化。其精细的无定形特性使其能够在电子束中精确保持。