Deak I I
J Morphol. 1977 Aug;153(2):307-16. doi: 10.1002/jmor.1051530209.
The thoracic muscles of Drosophila melanogaster can be classified into two classes, the fibrillar and the tubular muscles, on morphological grounds. Histochemical techniques were used to characterize these two classes of muscle according to their content of various enzymes (alpha-glycerophosphate, NAD-dependent isocitrate, malate and succinate dehydrogenases, fumarase, acid phosphatase, adenosine triphosphatase and acetylcholinesterase) and of glycogen. These investigations showed that the two muslces types are histochemically very different and, further, that the morphologically similar tubular muscles are heterogeneous with respect to their enzyme content. In particular, the tergal depressor of the trochanter of the second leg, the largest of the tubular muslces, has considerably less of all the enzymes studied, with the exception of acetylcholinesterase, than all the other tubular muscles examined. The histochemical techniqes were also used to follow the changes in enzyme levels that occur during development of the indirect flight muscle fibres. All the enzymes that are present in adult flight muslces showed an increase in staining intensity throughout muscle development. Some minor differences were observed in the time of appearance and rate of increase of intensity of the different enzymes.
基于形态学依据，果蝇的胸部肌肉可分为两类，即纤维状肌肉和管状肌肉。采用组织化学技术，根据这两类肌肉中各种酶（α - 甘油磷酸脱氢酶、NAD 依赖的异柠檬酸脱氢酶、苹果酸脱氢酶、琥珀酸脱氢酶、延胡索酸酶、酸性磷酸酶、三磷酸腺苷酶和乙酰胆碱酯酶）以及糖原的含量对其进行表征。这些研究表明，这两种肌肉类型在组织化学上差异很大，此外，形态相似的管状肌肉在酶含量方面也存在异质性。特别是，第二腿转子的背板降肌是最大的管状肌肉，除乙酰胆碱酯酶外，在所研究的所有酶中，其含量都比所有其他检查过的管状肌肉少得多。组织化学技术还用于追踪间接飞行肌纤维发育过程中酶水平的变化。成年飞行肌肉中存在的所有酶在整个肌肉发育过程中染色强度都有所增加。在不同酶出现的时间和强度增加的速率方面观察到了一些细微差异。
