Alarcón de Noya B, Torres J R, Noya O
Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela, Caracas.
Int J Parasitol. 1992 Sep;22(6):835-8. doi: 10.1016/0020-7519(92)90135-8.
Successful in vitro and in vivo maintenance of Sparganum proliferum is described for the first time. Various experimental animals including hamsters, mice and a monkey were evaluated. Albino mice inoculated either subcutaneously or intraperitoneally allowed the survival and multiplication of larvae for as long as 72 weeks. Intensity of infection was proportional to the length of exposure; however, the number of larvae collected from inoculated animals varied widely when infection lasted for 6 or more months. Inoculation of single larval segments appears as effective as that of complete larvae. Although Minimal Essential Medium allowed the survival of S. proliferum for as long as 14 weeks, growth was observed only during the first 4 weeks of culturing. Despite initial in vitro growth of larvae, neither differentiation into a more developed stage nor multiplication was obtained.
首次描述了多房棘球绦虫在体外和体内的成功维持。对包括仓鼠、小鼠和一只猴子在内的各种实验动物进行了评估。皮下或腹腔接种的白化小鼠可使幼虫存活并繁殖长达72周。感染强度与暴露时间成正比；然而，当感染持续6个月或更长时间时，从接种动物收集的幼虫数量差异很大。接种单个幼虫节段似乎与接种完整幼虫一样有效。尽管最低必需培养基可使多房棘球绦虫存活长达14周，但仅在培养的前4周观察到生长。尽管幼虫最初在体外生长，但未获得向更发育阶段的分化或繁殖。
