Blake J, Woodworth B A, Litzi-Davis L, Cosand W L
Bristol-Myers Squibb, Seattle, WA.
Int J Pept Protein Res. 1992 Jul;40(1):62-5. doi: 10.1111/j.1399-3011.1992.tb00105.x.
During the solid-phase synthesis of over 100 peptides, we have observed that the ethylcarbamoyl group is useful for the side chain protection of cysteine in peptides containing a single cysteine residue. The ethylcarbamoyl group is stable to the conditions of acidolytic cleavage, purification and long term storage. Brief treatment of peptides containing an S-ethylcarbamoyl-cysteine residue with aqueous sodium hydroxide gives the deprotected cysteine peptide that can be coupled to carrier molecules such as proteins to give immunogen conjugates.
在超过100种肽的固相合成过程中，我们观察到乙基氨基甲酰基对于含有单个半胱氨酸残基的肽中半胱氨酸的侧链保护是有用的。乙基氨基甲酰基在酸解切割、纯化和长期储存条件下是稳定的。用氢氧化钠水溶液对含有S-乙基氨基甲酰基-半胱氨酸残基的肽进行短暂处理，可得到脱保护的半胱氨酸肽，该肽可与蛋白质等载体分子偶联以得到免疫原缀合物。
