Tripathi R C, Kolli S P, Borisuth N S, Tripathi B J
Department of Ophthalmology and Visual Science, University of Chicago, Illinois 60637.
Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3449-54.
The authors identified and quantified the receptor for transferrin on trabecular meshwork (TM) cells cultured from porcine eyes by using two receptor assays. Flow-cytometric analysis of TM cells that were incubated with a monoclonal antibody to the transferrin receptor revealed such receptors, which decreased in number as the cells passed from the pre- to the post-confluent phase. Quantitative characterization by radioligand binding of 125I-transferrin to trabecular cells followed by Scatchard analysis showed that pre-confluent cultures expressed 23,839 +/- 6746 high-affinity receptors/cell and post-confluent cultures expressed 5104 +/- 3639 receptors/cell. The expression of the receptor for transferrin reflects the index of mitotic activity and can be correlated with the proliferative state of the trabecular cells. Further characterization of the receptors for transferrin in the TM may open up the possibility of a pharmacologic approach that enhances the TM cell population, which is known to decrease with age and in glaucomatous states of the eye.
作者通过两种受体检测方法，鉴定并定量了从猪眼中培养的小梁网（TM）细胞上的转铁蛋白受体。用抗转铁蛋白受体单克隆抗体孵育TM细胞后进行流式细胞术分析，结果显示存在此类受体，其数量在细胞从汇合前期进入汇合后期时减少。通过125I-转铁蛋白与小梁细胞的放射性配体结合进行定量表征，随后进行Scatchard分析，结果表明汇合前期培养物中每个细胞表达23,839±6746个高亲和力受体，汇合后期培养物中每个细胞表达5104±3639个受体。转铁蛋白受体的表达反映了有丝分裂活性指数，并且可以与小梁细胞的增殖状态相关联。对TM中转铁蛋白受体的进一步表征可能会开辟一种药理学方法的可能性，这种方法可以增加已知会随着年龄增长和眼部青光眼状态而减少的TM细胞数量。
