Rutz H P, Calmès J M, Burri F, Givel J C, Mirimanoff R O
Laboratoire de radiobiologie, Centre hospitalier universitaire vaudois (CHUV), Lausanne.
Helv Chir Acta. 1992 Aug;59(2):355-8.
Flow cytometry offers a relatively simple method to determine the DNA content of colorectal cancers. However, the technique can not discover minor changes in karyotype which may characterize near-diploid tumors, nor will it detect very small populations of aneuploid cells. In an attempt to improve the detection of such cells, we incubated biopsy material prior to analysis. This method revealed an aneuploid subpopulation in one of two biopsies studied. This simple protocol may ultimately lead to a more accurate assessment of the ploidy in colon cancer.
流式细胞术提供了一种相对简单的方法来测定结直肠癌的DNA含量。然而，该技术无法发现可能表征近二倍体肿瘤的核型微小变化，也无法检测到极少量的非整倍体细胞群体。为了提高对此类细胞的检测能力，我们在分析前对活检材料进行了孵育。该方法在研究的两份活检样本中的一份中发现了一个非整倍体亚群。这个简单的方案最终可能会导致对结肠癌倍性的更准确评估。
