通过用转染的L细胞免疫小鼠获得的识别HLA - DQ多态性的鼠单克隆抗体的制备与鉴定

Production and characterization of murine monoclonal antibodies recognizing HLA-DQ polymorphisms obtained by immunizing mice with transfected L cells.

作者信息

Vitale M, Pistillo M P, Tazzari P L, Falco M, Sun P F, Mantero S, Ferrara G B

机构信息

National Institute for Cancer Research, Genoa, Italy.

出版信息

Hum Immunol. 1992 Jun;34(2):126-34. doi: 10.1016/0198-8859(92)90038-o.

DOI:10.1016/0198-8859(92)90038-o

PMID:1429033

Abstract

Two polymorphic anti-HLA-DQB1 mAbs, TM 902 and TM 903, have been produced by immunizing F1 mice (Balb/C x C3H) with HLA-DQ-transfected mouse L cells. Cytotoxic analysis on a panel of HLA-typed cell lines has shown that TM 902 reacts with all the DQB1* alleles except DQB1*0501, *0502, and 0503, and DQB10601, *0602, *0603, and 0604, whereas TM 903 reacts with the DQB10501, *0502, and 0503, DQB10601, *0602, *0603, and 0604, and DQB10401 and *0402 alleles. The same reactivity pattern has been confirmed by cytofluorimetric analysis. Indirect immunofluorescence with various class-II-transfected cell lines showed no binding of both mAbs to the DR or DP products, suggesting their reactivity to the DQ products. The use of transfectants expressing HLA-DR/DQ heterodimers demonstrates that TM902 and TM903 mAbs are both specific for the DQ-beta chain. Comparison of the amino acid sequences of the DQ-beta chain suggests the involvement of residues 84-90 (QLELRTT) in the formation of TM902 epitope and of residues 54-55 (GR) in the formation of TM903 epitope.

摘要

通过用 HLA - DQ 转染的小鼠 L 细胞免疫 F1 小鼠（Balb/C×C3H），制备了两种多态性抗 HLA - DQB1 单克隆抗体 TM 902 和 TM 903。对一组 HLA 分型细胞系的细胞毒性分析表明，TM 902 与除 DQB1*0501、*0502、0503 以及 DQB10601、*0602、0603 和 0604 之外的所有 DQB1等位基因反应，而 TM 903 与 DQB10501、*0502、0503、DQB10601、*0602、*0603 和 0604 以及 DQB10401 和 *0402 等位基因反应。细胞荧光分析证实了相同的反应模式。用各种 II 类转染细胞系进行间接免疫荧光显示，两种单克隆抗体均不与 DR 或 DP 产物结合，表明它们对 DQ 产物有反应性。使用表达 HLA - DR/DQ 异二聚体的转染子表明，TM902 和 TM903 单克隆抗体均对 DQ - β链具有特异性。DQ - β链氨基酸序列的比较表明，84 - 90 位残基（QLELRTT）参与 TM902 表位的形成，54 - 55 位残基（GR）参与 TM903 表位的形成。