Tsujiuchi T, Tsutsumi M, Uchida K, Honoki K, Kondoh S, Denda A, Miwa M, Konishi Y
Department of Oncological Pathology, Nara Medical College.
Jpn J Cancer Res. 1992 Sep;83(9):985-8. doi: 10.1111/j.1349-7006.1992.tb02011.x.
The possibility of poly(ADP-ribosyl)ation playing a role during liver regeneration induced by partial hepatectomy (PH) in vivo was examined. When rats were given an i.p. injection of 3-aminobenzamide (ABA) at a dose of 600 mg/kg body weight 12 h after PH, the levels of DNA synthesis at 20 h after PH were significantly reduced. The time course of DNA synthesis in regenerating liver was significantly delayed in the ABA-treated group. Enzymatic assay revealed the activity of poly-(ADP-ribose)polymerase (PADPRP) in controls to be increased in parallel with the increase of DNA synthesis induced by PH. This increase in PADPRP activity was delayed and very much weaker after ABA treatment. The results thus suggested that poly(ADP-ribosyl)ation might play an important role in DNA synthesis during liver regeneration in vivo.
研究了体内部分肝切除（PH）诱导的肝再生过程中多聚（ADP - 核糖）化发挥作用的可能性。当大鼠在PH术后12小时腹腔注射剂量为600mg/kg体重的3 - 氨基苯甲酰胺（ABA）时，PH术后20小时的DNA合成水平显著降低。ABA处理组再生肝中DNA合成的时间进程显著延迟。酶活性测定显示，对照组中多聚（ADP - 核糖）聚合酶（PADPRP）的活性随着PH诱导的DNA合成增加而平行升高。ABA处理后，PADPRP活性的这种增加延迟且非常微弱。因此，结果表明多聚（ADP - 核糖）化可能在体内肝再生过程中的DNA合成中发挥重要作用。
