Foucault P, Carreau S, Kuczynski W, Guillaumin J M, Bardos P, Drosdowsky M A
Laboratoire de Biochimie, Centre Hospitaliere Universitaire Côte de Nacre, Caen, France.
J Androl. 1992 Sep-Oct;13(5):361-7.
Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxanthine; MIX). Testicular tissues were obtained from 10 young patients (mean age, 29 years); using a 3-step enzymatic treatment, Sertoli cell enriched preparations (> 92%) were studied after 4 days as primary cultures. No significant changes in lactate, estradiol-17 beta, and transferrin outputs have been observed according to age in patients ranging in age from 16 years to 47 years. Sertoli cell production of the compounds is controlled by testosterone plus MIX; FSH (or dbcAMP) treatment only slightly improves their synthesis. It is suggested that human Sertoli cell function, as far as the parameters measured in this study are concerned, is likely regulated by cAMP-dependent and independent pathways.
在存在或不存在睾酮加磷酸二酯酶抑制剂（1-甲基-3-异丁基黄嘌呤；MIX）的情况下，将人卵泡刺激素（FSH）或二丁酰环磷腺苷（dbcAMP）与人支持细胞共同孵育24小时后，测定了人支持细胞参数，即乳酸、雌二醇-17β和转铁蛋白的产生。从10名年轻患者（平均年龄29岁）获取睾丸组织；采用三步酶处理法，在4天后将富集支持细胞的制剂（>92%）作为原代培养物进行研究。在年龄范围为16岁至47岁的患者中，未观察到乳酸、雌二醇-17β和转铁蛋白产量随年龄有显著变化。这些化合物的支持细胞产生受睾酮加MIX控制；FSH（或dbcAMP）处理仅略微改善其合成。就本研究中测量的参数而言，提示人支持细胞功能可能受cAMP依赖性和非依赖性途径调节。
